"Quick Lessons" in Immunohematology

Quick Lessons in Immunohematology have been created to provide information on a variety of immunohematology topics in an easy-to-digest format.  Whether you are learning the fundamentals of blood banking, or would like a refresher on a specific subject, feel free to browse and download our current selection of Quick Lessons.  

Alloimmunization & Sickle Cell Disease


  1. Discuss alloimmunization rates for Sickle Cell Disease (SCD) patients compared to other groups of patients. 
  2. Describe the benefits and challenges of antigen-matching protocols for SCD patients.
  3. Explain how a SCD patient might develop alloantibodies to an antigen present on his/her cells. 

Antibody to a High Prevalence Antigen


  1. Describe characteristic serologic reactivity of an antibody to a high prevalence antigen.
  2. Explain how testing phenotype-matched RBCs differentiates multiple common antibodies from an antibody to a high prevalence antigen.
  3. Discuss how the following factors provide clues in the identification of an antibody to a high prevalence antigen: patient ethnicity, testing treated reagent RBCs, antigen typing the patient’s cells, testing rare cells.



  1. Describe the interference of anti-CD38 monoclonal antibody in blood bank tests.

  2. List ways to mitigate the serologic interference of anti-CD38.

  3. Discuss transfusion strategies for patients receiving anti-CD38 therapy. 

Basic Rh


  1. Discuss Rh haplotypes, using both Fisher-Race and Weiner nomenclature, and explain how to determine f status using other Rh typings.
  2. Compare and contrast weak D and partial D. 
  3. List unique circumstances in which an antibody might have Rh specificity.

Blood Bank Methodologies


  1. Describe tube testing, gel testing and solid phase testing in the blood bank.
  2. Compare and contrast the three methodologies.
  3. List advantages and disadvantages of each methodology.

Elution & Eluates


  1. List several reasons why an individual might have a positive direct antiglobulin test (DAT).
  2. Explain the process of preparing an acid eluate.
  3. Discuss how the reactivity of an eluate can explain the cause of a positive DAT. 

Platelet Antibodies


  1. Name three types of antigens expressed on the surface of platelets.
  2. Discuss immune platelet refractoriness, including antibodies that cause refractoriness and transfusion options.
  3. Describe two conditions caused by antibodies to human platelet antigens (HPA).



  1. Discuss the most common reasons titration is used in the blood bank.
  2. Discuss the reactivity known as HTLA.
  3. Interpret titer results.